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. Author manuscript; available in PMC: 2011 Feb 10.
Published in final edited form as: Nano Lett. 2010 Feb 10;10(2):719–725. doi: 10.1021/nl903968s

Figure 4.

Figure 4

Fluorescent immunostaining to confirm the presence and functionality of patterned fibronectin. (a) Differential contrast microscopy image of the original parylene template, with arrays of 180nm lines marked by the arrow. (b) Fluorescence microscopy image showing immunostaining of the fibronectin features successfully patterned onto the surface. The image brightness and contrast have been adjusted, so that the dim fluorescence of the lines demarcated by the arrow could be seen. The arrays of 90nm spots could not be easily visualized by optical microscopy since their size is significantly smaller than the diffraction limit.