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Western blot showing expression of syt IV (above) and syt I (below). Expression in PC12 cells is shown on the right; standards with recombinant protein are on the left. The syt IV signal was ∼2.5 times higher in syt IV overexpressing than control cells. Accounting for the fraction of cells transfected gives 12.5-fold higher syt IV levels in transfected cells. Syt I levels were indistinguishable in control and syt IV overexpressing cells.
