Fusion pore reconstruction for kiss-and-run events. The imaginary (Im) and real (Re) lock-in outputs were used to compute conductance traces (see Methods). (A) Fusion pore reconstruction in control cells (left, DCVs; right, MVs). (B) Fusion pore reconstructions in syt IV cells (left, DCVs; right, MVs). (C) DCV fusion pore duration (left), conductance (middle), and radius (right) were compared between control and syt IV cells. (D) MV fusion pore duration (left), conductance (middle), and radius (right) were compared between control and syt IV cells. Stars indicate statistical significance (p < 0.05).