Figure 1.
(A) Calorimetric transitions for thioredoxin denaturation in the presence of several thermolysin concentrations (shown in mg/mL as the numbers alongside the transitions). See the Supporting Material for details about calorimetric experiments and thermogram processing. (Inset) plot of transition temperature (maximum of the transition) versus log10 of thermolysin concentration. (B) Illustrative plots of rate constant for irreversible denaturation versus thermolysin concentration for the temperatures shown. Rates constant values are calculated from the DSC transitions (see text and the Supporting Material for details) and the lines are the best fits of Eq. 3.