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Sequestering of full length HA from contact regions. HA was labeled with TMR and reconstituted into GUVs made of DOPC/DOPE/DOPS (3:1:1, mol/mol/mol), containing 1 mol % N-NBD-PE. On addition of 2 mM Ca2+ adhesion of GUVs and formation of regions depleted of HA could be observed. (A) TMR-HA and (B) N-NBD-PE fluorescence. See the Supporting Material for details. Scale bar = 5 μm.
