Table 1.
Lysozyme hydration, h, determined by different methods
aw = 0.75 |
aw = 0.91 |
aw = 0.95 |
||||
---|---|---|---|---|---|---|
h (g/g ± SD) | N | h (g/g ± SD) | N | h (g/g ± SD) | N | |
Hydration | ||||||
Bulk vapor sorption | 0.18 ± 0.03 | 6 | 0.28 ± 0.03 | 6 | 0.52 ± 0.04 | 9 |
Sorption calorimetry∗ | 0.18 | — | 0.29 | — | 0.41 | — |
Dehydration | ||||||
Decanol dehydration† | 0.24 ± 0.03 | 17 | 0.40 ± 0.05 | 13 | 0.54 ± 0.09 | 13 |
Single-particle vapor sorption | 0.28 ± 0.06 | 2 | 0.39 ± 0.05 | 4 | 0.54 ± 0.08 | 3 |
Bulk vapor sorption | 0.20 ± 0.01 | 4 | 0.40 ± 0.04 | 7 | 0.51 ± 0.03 | 7 |
Sorption calorimetry∗ | 0.24 | — | 0.47 | — | 0.51 | — |
Sorption calorimetry data taken from Fig. 3 in Kocherbitov et al. (29).
Water activities calculated from f = 0.51, 0.75, and 0.85, are 0.76, 0.91, and 0.96, respectively.