Table 1.

ATP-induced distance changes between symmetry-related spin labels in NABB-reconstituted MsbA

		Experimental distance Rav (Å), NABB			Calculated distance ΔRCα- Cα (Å)
Mutant	Location	Apo	ADP-Vi	ΔRav	Open	AMPPNP	ΔRCα- Cα
158	TM3	39.5 ± 3	45.0 ± 6	−5.5	41	46	−5
143		49.5 ± 4	30.0 ± 2	19.5	47	28	19
115	IH1	54.0 ± 5	32.0 ± 1	22	62	31	31
561	NBD	>70	42.0 ± 2	>28	86	36	50