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Perturbation of the ClC-ec1 system along the lowest-frequency NM, viewed from within a membrane plane. The feature identification scheme of Fig. 1 is used. (A) The minimized system. (B and C) “Positive” and “negative” displacement along the seventh all-atom NM. Pore lining residues are suppressed. The oval and triangles highlight the protein regions most affected during perturbation.
