Table 3.
Drosophila TDT muscle fiber mechanics as measured by force-clamp analysis
a/Tmax (no units) | b (ML/s) | Vmax (ML/s) | Vopt (ML/s) | Topt/Tmax (%) | pmax (ML/s × T/Tmax) | pmax (ML/s × mN/mm2) | |
---|---|---|---|---|---|---|---|
Tr-WT | 0.15 ± 0.02 | 0.77 ± 0.10 | 5.10 ± 0.34 | 1.34 ± 0.08 | 26 ± 1 | 0.35 ± 0.03 | 12.9 ± 1.4 |
EMB | 0.14 ± 0.03 | 0.43 ± 0.04∗ | 3.23 ± 0.38∗ | 0.85 ± 0.06∗ | 25 ± 1∗ | 0.20 ± 0.01∗ | 11.3 ± 1.4 |
a, fitted parameter based on Hill's equation; b, fitted parameter based on Hill's equation; ML, muscle length; pmax, maximum power; Tmax, maximum activated tension; Topt, tension at maximum power; Tr-WT, wild-type myosin isoform; Vmax, maximum shortening velocity extrapolated from the Hill fit of tension-velocity curves; Vopt, velocity at maximum power.
The pmax values in the final column were calculated using Vopt, Topt/Tmax, and Tmax from Table 2. n = 10 for both Tr-WT and EMB. Values are mean ± SE.
Significantly different from Tr-WT, p < 0.05, Student's t-test.