Figure 3.

Analysis of anti-His₆ antibody binding to His₈-tagged ASIC1a. (A) Low-magnification AFM images of a sample of isolated ASIC1a that had been incubated with anti-His₆ antibody. Arrowheads indicate singly decorated ASICs; arrows indicate doubly decorated ASICs. A shade-height scale is shown at the right. (B) Gallery of zoomed images of ASICs that are undecorated (top) or decorated by one (middle) or two (bottom) anti-His₆ antibodies. Lines indicate the angles between pairs of bound antibodies. (C–E) Frequency distributions of (C) molecular volumes of decorated central particles, (D) molecular volumes of bound peripheral particles, and (E) angles between pairs of bound peripheral particles. The curves indicate the fitted Gaussian functions. The means of the distributions are indicated.