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Determination of the raft association of ASIC1a and the σ-1 receptor. Cells were extracted with ice-cold Triton X-100 (1%) and subjected to centrifugation on Optiprep density gradients. Fractions from the gradient were analyzed by SDS-PAGE and immunoblotting using anti-ASIC1a antibody, anti-FLAG antibody (for the σ-1 receptor), or anti-caveolin antibody (as a raft marker).
