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. Author manuscript; available in PMC: 2010 Apr 5.
Published in final edited form as: Chembiochem. 2008 Jan 25;9(2):286–293. doi: 10.1002/cbic.200700489

Figure 2.

Figure 2

Dependence of CF release on zinc complexation to form transport active 6. Apo-6 (1 µM) was added at 50 s to POPC vesicles (25 µM). Inside the vesicles is 5 mM TES, 50 mM CF, 100 mM NaCl (pH 7.4) buffer; outside the vesicles is 5 mM TES, 100 mM NaCl (pH 7.4) buffer. Zn(NO3)2 (0 µM (■), 0.25 µM (×), 0.5 µM (●), 1 µM (◆) and 2 µM (◇)) was added at 100 s and the vesicles lysed with Triton X-100 at 750 s.