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. 2010 Apr 1;24(7):647–652. doi: 10.1101/gad.1881710

Figure 2.

Figure 2.

In vivo screen suggests that the essential mst1 HAT (TIP60 homolog) and the sirtuin sir2 HDAC are the major enzymes that catalyze H3K4ac and H3K4 deacetylation, respectively. (A) Western blot analysis of WCEs from cells of the indicated genotypes probed with a specific anti-H3K4ac (Supplemental Figs. S3–5) and control anti-H3K9ac and anti-H3K56ac antibodies. (B) IF with anti-H3K4ac antibody of mst1-ts cells exponentially grown at the permissive temperature (25°C) or shifted for 2 h at the nonpermissive temperature (36°C). (C) Western blot analysis with anti-H3K4ac antibody of wild-type (WT) or mst1-ts cells exponentially grown at the permissive temperature (25°C) or shifted for 4 h at the semipermissive (32°C) and nonpermissive (36°C) temperatures. (D) Western blot analysis with anti-H3K4ac and anti-H3K9ac antibodies of in vitro HAT assays with Mst1- and GST-tagged TIP60 using recombinant histone hH3.1 wild type or H3K4R as substrate. Mst1 was purified from SF9 insect cells infected with a bacculovirus containing mst1 ORF under a polyhedrin promoter.