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. 2010 Apr 1;24(7):683–695. doi: 10.1101/gad.560310

Figure 3.

Figure 3.

Tlx overexpression-induced NSC expansion is not a transient effect. (A) Coronal sections from aged animals as indicated stained with Ki67. Note Ki67-positive cells outside of the SVZ in 2-yr-old Tlx-OE mice but not in wild-type mice, and in transgenic animals at the age of 17 mo. Bar, 100 μm. (B) Coronal sections from aged animals as indicated stained with DCX. Note that more DCX-positive cells migrate out of the SVZ in the aged Tlx-OE mice, and more DCX-positive cells are found in the 2-yr-old Tlx-OE mice compared with 17-mo-old Tlx-OE mice (arrows). Bar, 50 μm. (C) Changes of Ki67-positive cells in the SVZ during aging. Note a decrease of cell proliferation in the SVZ of both wild-type and Tlx-OE mice. However, the number of Ki67-positive cells is significantly higher in Tlx-OE mice in all age groups (n = 3, P < 0.05, mean ± standard deviation [SD]), with aged Tlx-OE mice showing Ki67 indices comparable with those of young wild-type mice. (D) Tlx staining of 24-mo-old SVZ of wild-type and Tlx-OE brains. Note that more Tlx-expressing cells are present in the Tlx-OE SVZ. (E) BrdU feeding experiment was performed on 24-mo-old animals, and mice were analyzed for BrdU and NeuN costaining 7 wk after withdrawal of BrdU. Bar, 20 μm.