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. 2010 Apr 1;24(7):696–707. doi: 10.1101/gad.1859310

Figure 5.

Figure 5.

Conditional deletion of CoupTFII using Prox1+/CreERT2 reduces the expression of LEC markers. Prox1+/CreERT2;CoupTFII+/f and Prox1+/CreERT2;CoupTFIIf/f embryos were exposed to 5 mg TM at E12.5 and were analyzed at E15.5 by immunostaining of sections with different LEC markers. (A) Prox1 (red) and Nrp2 (green) are coexpressed in the LECs of a peripheral lymphatic vessel of Prox1+/CreERT2;CoupTFII+/f embryos. (B) However, in Prox1+/CreERT2;CoupTFIIf/f embryos, these vessels are dilated and mispatterned, and the expression of Nrp2 is substantially reduced. (C) Prox1 (green) and Lyve1 (red) are coexpressed in the peripheral lymphatic vessel (arrow) of Prox1+/CreERT2;CoupTFII+/f embryos. (D) In contrast, the lymphatic vessels of Prox1+/CreERT2;CoupTFIIf/f embryos are dilated, and Lyve1 expression is down-regulated in the Prox1+ LECs of the peripheral lymphatic vessels (arrows). The scattered Prox1−Lyve1+ cells are macrophages. (E,F) Deletion of the floxed Coup-TFII allele results in the activation of the LacZ reporter gene that expresses β-gal (red). (E) Costaining with the LEC marker podoplanin (green) shows that these markers are coexpressed in the lymphatic vessels of Prox1+/CreERT2;CoupTFII+/f embryos. (F) In contrast, the expression of podoplanin is substantially reduced in the lymphatic vessels of Prox1+/CreERT2;CoupTFIIf/f embryos. The neural tube is oriented toward the bottom of A, and toward the right side of B–F. Bar, 50 μm.