Saliva was collected as described in the Materials and Methods section over a 20-min period after stimulation with either 0.35 mg/kg BW (A, B, C) or 0.15mg/kg BW (D) pilocarpine. Animals ranged in age from 7–20 weeks. For this experiment, 28 male and 20 female SJL mice were used. Female and male SJL IL-12 TG mice show decreased salivary flow (A). Change in salivary flow in female mice (B) is not age or dose (D) dependent. On the contrary, male mice (C) showed an age dependent change in salivary flow. Horizontal bars are mean values for each group. Significant differences are indicated (*) and were determined by non-parametric Wilcoxon's ranksum test. WT = wild type, TG = IL-12 transgenic.