Each row in panels A-I shows a stage 8 egg chamber from a female maintained under conditions that promote formation of the reticulated bodies. Double labeling was used to assess protein colocalization.
A-C: Me31B∷GFP (B and green in A) and Dcp1 (C and red in A). Dcp1 is highly concentrated in the reticulated bodies.
D-F: Me31B∷GFP (E and green in D) and PABP (F and red in D). PABP is dispersed throughout the cytoplasm (with a high concentration in follicle cells, which encompass the egg chamber) and is not enriched in reticulated bodies (the arrows point to one of the bodies).
G-I: Orb (H and red in G) and ER-EYFP (I and green in G). The reticulated bodies do not exclude ER (the arrows point to one of the bodies). This egg chamber shows the more extreme type of reticulated bodies. Scale bars are 20 μm in all panels.