FIG. 4.

FA profiles of B. cereus WT and ΔBC5401 strains at two temperatures. The WT (black bars) and the ΔBC5401 strain (gray bars) were grown at 37°C (solid bars) or 12°C (striped bars) on LB agar. The profiles of FAMEs were determined by GC-MS as described previously (39). The areas of the detected peaks (each corresponding to a distinct FA) were measured. Each bar represents the relative amount of one FA compound, expressed as the area of the individual peak divided by the sum of the areas of all the peaks on the chromatogram (mean ± SEM of results for triplicate biological samples measured twice). Only data for major FA compounds (i.e., FAs for which the area of the individual peak was >2% of the total area at either 37 or 12°C) are presented. For a given FA, the presence of the same letters above the bars indicates that no significant difference between results for the two temperatures and/or strains was detected (P > 0.01; Tukey's HSD test).