TABLE 2.
Primers used in this study
| Primer name | 5′-3′ sequencea | Restriction site |
|---|---|---|
| 5Up-01_Bam | CGGGATCCATAGCGCTATTATCGAGGGG | BamHI |
| 3Up-01_Pst | AAAACTGCAGCGCCCAACATAGCCAATTCC | PstI |
| 5Dn-01_Xba | GCTCTAGAACAAGGAGGGGTAAAATGACG | XbaI |
| 3Dn-01_Eag | CGCGGCCGGCCTTCTACACTCACTTCCC | EagI |
| 5Up-02_Bam | CGGGATCCGCGAACTATCGAATTGAAGGG | BamHI |
| 3Up-02_Pst | AAACTGCAGGCTGTCTCTTTTGATATCCCC | PstI |
| 5Dn-02_Xba | GCTCTAGAGCGGGGATGGTTACAGAAGC | XbaI |
| 3Dn-02_Eag | CGCGGCCGCTCTGTGTCAGGACGTACG | EagI |
| Km5in | TCTGTCTAGACATTTGAGGTGATAGG | XbaI |
| Km3in | GCTACTGCAGATCGATACAAATTCCTCGTAGGCG | PstI |
| Km5out | CGGTATAATCTTACCTATCACC | |
| Km3out | TACTCTGATGTTTTATATCTTTTCTAA | |
| IVET-I1 | CCCTGAACAGTGTTCTCGG | |
| IVET-I2 | GGCGATTAAGTTGGGTAAC | |
| RT-5402-F | TTATTGCCACACGTAAATGA | |
| RT-5402-R | CCCACGTGAAAATACTTTGT | |
| RT-5402-01-F | ACAAGGAGTTCGTATTCCAG | |
| RT-5402-01-R | GCCAATTCCTTCTTTATCAC | |
| RT-5401-F | GATGTAGTGGCAAAAGAGAA | |
| RT-5401-R | CAGGACGATACGTCTCTAAA | |
| RT-5401-00-F | AGCGCTAAACGATAAAAATG | |
| RT-5401-00-R | TCTGGGTTTTCAACGATACT | |
| RT-5400-F | CGAATGTTAGTCGGATTGAT | |
| RT-5400-R | TAATCCCTGCTGGATCTAAA | |
| RT-5400-5399-F | GAAGGCGATCGATTACTATG | |
| RT-5400-5399-R | CGGTTCTGAGAATCAACAAT | |
| RT-5399-98-F | AGGTTTATCGATGGGATTTT | |
| RT-5399-98-R | CAGAAAAGCTTAAACCATGC | |
| RT-5398-F | TTGTCATTTTAGGGCAAGAT | |
| RT-5398-R | CCCCCACAATATGAAAATAA | |
| LC-BC5402-F | TTATTGCCACACGTAAATGA | |
| LC-BC5402-R | CCCACGTGAAAATACTTTGT | |
| LC-BC5401-F | CATATGGCTTTGTTTCAGG | |
| LC-BC5401-R | TTTGCACCACTAACTGCTAA | |
| LC-16S-F | GGTAGTCCACGCCGTAAACG | |
| LC-16S-R | GACAACCATGCACCACCTG |
a
Restriction enzyme sites are underlined.