TABLE 4.
B. cereus genes identified in this study as being specifically expressed during growth at low temperatures
| Clone no.a | Insert size (bp) | No. of potential promoters | Downstream ORFb |
COG category(ies)c | Broad COG categoryd | Assigned functional description of gene producte | |
|---|---|---|---|---|---|---|---|
| NCBI no. | ERGO no. | ||||||
| CIP60 | 719 | 1 | BC0544 (+3) | RZC04345 | C | Metabolism | Iron-sulfur cluster-binding protein |
| CIP81 | 711 | 1 | BC0749 (+6) | RZC02927 | H | Metabolism | Thiazole biosynthesis protein ThiG |
| CIP16 | 1,473 | 2 | BC0795 | RZC01484 | O | Cellular processes and signaling | Molybdopterin biosynthesis MoeB protein |
| BC0796 | RZC01487 | P | Metabolism | Rhodanese-related sulfurtransferase | |||
| CIP30 | 558 | 1 | BC1235 (+6) | RZC03344 | E | Metabolism | Indole-3-glycerol phosphate synthase |
| CIP50 | 1,083 | 1 | BC1647 (+8) | RZC03052 | NU | Cellular processes and signaling | Flagellar biosynthesis/type III secretory pathway ATPase |
| CIP13 | 1,931 | 1 | BC2489 | RZC06907 | I | Metabolism | Acyl-CoA synthetase/AMP-(fatty) acid ligase |
| CIP47 | 1,536 | 2 | BC3197 | RZC04150 | I | Metabolism | Permease of the major facilitator superfamily |
| BC3196 | RZC07700 | GEPR | Metabolism | Biotin carboxylase | |||
| CIP49 | 1,155 | 3 | BC4246 | RZC02388 | * | Poorly characterized | Hypothetical protein |
| BC4247 | RZC02389 | * | Poorly characterized | Hypothetical protein | |||
| BC4248 | RZC02393 | * | Poorly characterized | Hypothetical protein | |||
| CIP25 | 1,315 | 1 | BC4634 | RZC01131 | * | Poorly characterized | Hypothetical protein |
| CIP7 | 498 | 1 | BC4851 | RZC00481 | IQ | Metabolism | o-Succinylbenzoic acid-CoA ligase |
| CIP18 | 1,166 | 1 | BC4866 | RZC03491 | G | Metabolism | Glc-1-P adenyltransferase |
| CIP85 | 641 | 1 | BC4943 | RZC07012 | * | Poorly characterized | Hypothetical protein |
| CIP54 | 963 | 1 | BC4977 (+2) | RZC02376 | F | Metabolism | 5′-Nucleotidase/2′,3′-cyclic phosphodiesterase or related esterase |
| CIP5 | 702 | 1 | BC5400 (+2) | RZC04931 | V | Cellular processes and signaling | Bacitracin transport ATP-binding protein BcrA |
| CIP22 | 858 | 1 | BC5401 (+3) | RZC04935 | E | Metabolism | Lipase/acylhydrolase with GDSL-like motif |
| CIP26 | 1,691 | 1 | BC5402 (+4) | RZC04938 | K | Information storage and processing | Transcriptional regulator in the LacI family |
| CIP39, CIP2, CIP1 | 1,187 | 1 | BC5440 | RZC06561 | KT | Information storage and processing | Autolysin response regulator |
The listing of three numbers together indicates that the same clone was identified in three independent screenings.
Numbers in parentheses correspond to the number of genes organized as an operon found downstream of the CIP. ERGO numbers are according to the database for the ERGO bioinformatics system.
Data are from Tatusov et al. (44). *, no COG.
Broad functional COG category according to the NCBI website (ftp://ftp.ncbi.nih.gov/pub/COG/COG/fun.txt).
Assigned functional description according to the B. cereus genome databases (http://www.integratedgenomics.com and http://www.ncbi.nlm.nih.gov). Boldface indicates products of genes on which this study focused further.