TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotypea | Description or relevant characteristic | Source |
|---|---|---|---|
| S. mutans strains | |||
| GS-5 | GlnR+ | Wild-type strain | H. K. Kuramitsu |
| ΔglnR mutant | Emr GlnR− | GS-5 strain lacking glnR | This study |
| CglnRΔglnR | Emr Sper GlnR+ | ΔglnR complementation strain | This study |
| Cit1-luc | Kmr GlnR+ | GS-5 chromosome harboring a single copy of pcit1-luc | This study |
| Cit2-luc | Kmr GlnR+ | GS-5 chromosome harboring a single copy of pcit2-luc | This study |
| Cit3-luc | Kmr GlnR+ | GS-5 chromosome harboring a single copy of pcit3-luc | This study |
| luc | Kmr GlnR+ | GS-5 chromosome harboring a single copy of the luc gene | This study |
| Plasmids | |||
| pDL277 | Sper | E. coli-Streptococcus shuttle vector | LeBlanc et al. (34) |
| pDL277-glnR | Sper | pDL277 harboring glnR | This study |
| pMC340B | Emr Kmr | Integration vector for S. mutans | Y.-Y. M. Chen |
| pMC340Bluc | Emr Kmr | pMC340B containing promoterless luc gene | This study |
| pcit1-luc | Emr Kmr | pMC340Bluc with 254-bp DNA fragment of the citB promoter | This study |
| pcit2-luc | Emr Kmr | pMC340Bluc with 101-bp DNA fragment of the citB promoter | This study |
| pcit3-luc | Emr Kmr | pMC340Bluc with 75-bp DNA fragment of the citB promoter | This study |
a
The r superscript indicates resistance to the drugs (erythromycin [Em], spectinomycin [Spe], and kanamycin [Km]).