TABLE 2.
KS amino acid sequencesa
| Strain | Nearest type strain | No. of clones sequenced | No. of unique clones | NCBI accession no. | Top BLAST match (source organism), clone identifiera | BLAST match pathway product | % identityb | Cluster | No. of clones of PKS typec: |
||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mod. | Iter. | Hyb. | |||||||||
| CNU-125 | Actinomadura cremea | 2 | 1 | FJ844611 | chlB1 (Streptomyces antibioticus) | Chlorothricin | 58 | 1 | 0 | 1 | 0 |
| CNJ-863 | Gordonia nitida | 26 | 1 | FJ844612 | mcyG KS1 (Microcystis aeruginosa), 1 | Microcystin | 45 | 1 | 0 | 0 | 1 |
| CNJ-878 | Micromonospora endolithica | 2 | 2 | FJ844613 | mtaB (Stigmatella aurantiaca) | Myxothiazol | 60 | 1 | 1 | 0 | 0 |
| FJ844614 | nysI (Streptomyces noursei) | Nystatin | 63 | 2 | 1 | 0 | 0 | ||||
| CNJ-859 | Mycobacterium brisbanense | 1 | 1 | FJ844615 | jamL (Lyngbya majuscula) | Jamaicamide | 49 | 1 | 1 | 0 | 0 |
| CNJ-823 | Mycobacterium poriferae | 2 | 1 | FJ844616 | chlA2 (S. antibioticus) | Chlorothricin | 75 | 1 | 1 | 0 | 0 |
| CNS-044 | Nocardia arthritidis | 13 | 4 | FJ844617 | amphC (Streptomyces nodosus) | Amphotericin | 71 | 1 | 1 | 0 | 0 |
| FJ844618 | fcsC (Streptomyces sp. strain FR-008) | Candicidin | 66 | 2 | 1 | 0 | 0 | ||||
| FJ844619 | jamJ (Lyngbya majuscula) | Jamaicamide | 51 | 3 | 1 | 0 | 0 | ||||
| FJ844620 | mcyD (Microcystis aeruginosa) | Microcystin | 52 | 4 | 1 | 0 | 0 | ||||
| CNR-923 | Nocardiopsis lucentensis | 3 | 1 | FJ844621 | chlB1 KS1 (S. antibioticus), 1 | Chlorothricin | 53 | 1 | 0 | 1 | 0 |
| CNS-051 | Salinispora arenicola | 2 | 1 | FJ844622 | chlB1 (S. antibioticus) | Chlorothricin | 61 | 1 | 0 | 1 | 0 |
| CNS-205 | Salinispora arenicola | 98 | 7 | FJ844623 | rifB KS2 (Amycolatopsis mediterranei), 1247 | Rifamycin | 87 | 1 | 1 | 0 | 0 |
| FJ844624 | rifB KS3 (Amycolatopsis mediterranei), 1247 | Rifamycin | 89 | 1 | 1 | 0 | 0 | ||||
| FJ844625 | rifE KS1 (Amycolatopsis mediterranei), 1250 | Rifamycin | 86 | 1 | 1 | 0 | 0 | ||||
| FJ844626 | merC KS1 (Streptomyces violaceusniger), 3152 | Meridamycin | 68 | 2 | 1 | 0 | 0 | ||||
| FJ844627 | chlB1 KS1 (S. antibioticus), 2029 | Chlorothricin | 75 | 3 | 0 | 1 | 0 | ||||
| FJ844628 | chlB1 KS1 (S. antibioticus), 2407 | Chlorothricin | 55 | 4 | 0 | 1 | 0 | ||||
| FJ844629 | chlB1 KS1 (S. antibioticus), 4951 | Chlorothricin | 60 | 5 | 0 | 1 | 0 | ||||
| CNS-143 | Salinispora pacifica | 45 | 5 | FJ844630 | vicA (Streptomyces halstedii) | Vicenistatin | 75 | 1 | 1 | 0 | 0 |
| FJ844631 | vicA (S. halstedii) | Vicenistatin | 77 | 1 | 1 | 0 | 0 | ||||
| FJ844632 | vicB (S. halstedii) | Vicenistatin | 72 | 1 | 1 | 0 | 0 | ||||
| FJ844633 | vicC (S. halstedii) | Vicenistatin | 71 | 1 | 1 | 0 | 0 | ||||
| FJ844634 | vicD (S. halstedii) | Vicenistatin | 72 | 1 | 1 | 0 | 0 | ||||
| CNS-055 | Salinispora pacifica A | 40 | 8 | FJ844635 | vicA (S. halstedii) | Vicenistatin | 73 | 1 | 1 | 0 | 0 |
| FJ844636 | vicA (S. halstedii) | Vicenistatin | 72 | 1 | 1 | 0 | 0 | ||||
| FJ844637 | vicB (S. halstedii) | Vicenistatin | 72 | 1 | 1 | 0 | 0 | ||||
| FJ844638 | vicD (S. halstedii) | Vicenistatin | 74 | 1 | 1 | 0 | 0 | ||||
| FJ844639 | vicD (S. halstedii) | Vicenistatin | 70 | 1 | 1 | 0 | 0 | ||||
| FJ844640 | merC (S. violaceusniger) | Meridamycin | 68 | 2 | 1 | 0 | 0 | ||||
| FJ844641 | mcyE (Microcystis aeruginosa) | Meridamycin | 50 | 3 | 1 | 0 | 0 | ||||
| FJ844642 | curG (Lyngbya majuscula) | Curacin | 50 | 4 | 0 | 0 | 1 | ||||
| CNS-237 | Salinispora pacifica B | 40 | 11 | FJ844643 | curI (Lyngbya majuscula) | Curacin | 57 | 1 | 1 | 0 | 0 |
| FJ844644 | ecoC (Streptomyces aizunensis) | ECO-02301 | 71 | 2 | 1 | 0 | 0 | ||||
| FJ844645 | ecoC (S. aizunensis) | ECO-02301 | 69 | 2 | 1 | 0 | 0 | ||||
| FJ844646 | ecoC (S. aizunensis) | ECO-02301 | 71 | 2 | 1 | 0 | 0 | ||||
| FJ844647 | ecoC (S. aizunensis) | ECO-02301 | 71 | 2 | 1 | 0 | 0 | ||||
| FJ844648 | ecoC (S. aizunensis) | ECO-02301 | 68 | 2 | 1 | 0 | 0 | ||||
| FJ844649 | ecoC (S. aizunensis) | ECO-02301 | 69 | 2 | 1 | 0 | 0 | ||||
| FJ844650 | ecoG (S. aizunensis) | ECO-02301 | 68 | 2 | 1 | 0 | 0 | ||||
| FJ844651 | spnD (Saccharopolyspora spinosa) | Spinosad | 75 | 3 | 1 | 0 | 0 | ||||
| FJ844652 | spnD (Saccharopolyspora spinosa) | Spinosad | 73 | 3 | 1 | 0 | 0 | ||||
| FJ844653 | mcyE (Microcystis aeruginosa) | Microcystin | 50 | 4 | 1 | 0 | 0 | ||||
| CNJ-927 | Serinicoccus marinus | 1 | 1 | FJ844654 | chlB1 KS1 (S. antibioticus), 1 | Chlorothricin | 53 | 1 | 0 | 1 | 0 |
| CNR-876 | Streptomyces aureofaciens | 1 | 1 | FJ844655 | jamK (Lyngbya majuscula) | Jamaicamide | 56 | 1 | 1 | 0 | 0 |
| CNR-881 | Streptomyces bikiniensis | 2 | 1 | FJ844656 | jamP (Lyngbya majuscula) | Jamaicamide | 54 | 1 | 0 | 0 | 1 |
| CNR-918 | Streptomyces caviscabies | 3 | 2 | FJ844657 | jamK (Lyngbya majuscula) | Jamaicamide | 54 | 1 | 1 | 0 | 0 |
| FJ844658 | chlB1 (S. antibioticus) | Chlorothricin | 52 | 2 | 0 | 1 | 0 | ||||
| CNR-924 | Streptomyces chartreusis | 3 | 2 | FJ844659 | ecoA (S. aizunensis) | ECO-02301 | 75 | 1 | 1 | 0 | 0 |
| FJ844660 | epoD (Polyangium cellulosum) | Epothilone | 68 | 2 | 1 | 0 | 0 | ||||
| CNR-872 | Streptomyces hebeiensis | 2 | 1 | FJ844661 | jamL (Lyngbya majuscula) | Jamaicamide | 59 | 1 | 1 | 0 | 0 |
| CNS-177 | Streptomyces lydicus | 3 | 2 | FJ844662 | fcsC (Streptomyces sp. FR-008) | Candicidin | 74 | 1 | 1 | 0 | 0 |
| FJ844663 | merC (S. violaceusniger) | Meridamycin | 72 | 2 | 1 | 0 | 0 | ||||
| CNJ-962 | Streptomyces sampsonii | 1 | 1 | FJ844664 | lipC (S. aureofaciens) | Lipomycin | 73 | 1 | 1 | 0 | 0 |
| CNR-887 | S. sampsonii | 1 | 1 | FJ844665 | conB (Streptomyces neyagawaensis) | Concanamycin | 71 | 1 | 1 | 0 | 0 |
| CNR-879 | Streptomyces tendae | 2 | 2 | FJ844666 | merB (S. violaceusniger) | Meridamycin | 70 | 1 | 1 | 0 | 0 |
| FJ844667 | vicA (S. halstedii) | Vicenistatin | 77 | 2 | 1 | 0 | 0 | ||||
| CNR-885 | S. tendae | 41 | 14 | FJ844668 | kijD KS2 (Actinomadura kijaniata), 1 | Kijanimicin | 89 | 1 | 1 | 0 | 0 |
| FJ844669 | kijA KS4 (A. kijaniata), 2 | Kijanimicin | 81 | 2 | 1 | 0 | 0 | ||||
| FJ844670 | kijA KS4 (A. kijaniata), 3 | Kijanimicin | 84 | 2 | 1 | 0 | 0 | ||||
| FJ844671 | kijC KS1 (A. kijaniata), 4 | Kijanimicin | 85 | 2 | 1 | 0 | 0 | ||||
| FJ844672 | kijC KS1 (A. kijaniata), 5 | Kijanimicin | 84 | 2 | 1 | 0 | 0 | ||||
| FJ844673 | kijA KS4 (Actinomadura kijaniata), 6 | Kijanimicin | 80 | 2 | 1 | 0 | 0 | ||||
| FJ844674 | kijB KS2 (A. kijaniata), 7 | Kijanimicin | 91 | 2 | 1 | 0 | 0 | ||||
| FJ844675 | merA KS3 (S. violaceusniger), 8 | Meridamycin | 76 | 3 | 1 | 0 | 0 | ||||
| FJ844676 | merA KS3 (S. violaceusniger), 9 | Meridamycin | 74 | 3 | 1 | 0 | 0 | ||||
| FJ844677 | spnA KS1 (Saccharopolyspora spinosa), 12 | Spinosad | 76 | 4 | 1 | 0 | 0 | ||||
| FJ844678 | spnA KS1 (Saccharopolyspora spinosa), 13 | Spinosad | 72 | 4 | 1 | 0 | 0 | ||||
| FJ844679 | ttmC KS1 (Streptomyces spiroverticillatus), 10 | Tautomycin | 93 | 5 | 1 | 0 | 0 | ||||
| FJ844680 | ttmC KS2 (S. spiroverticillatus), 11 | Tautomycin | 95 | 5 | 1 | 0 | 0 | ||||
| FJ844681 | kijA KS2 (A. kijaniata), 14 | Kijanimicin | 72 | 6 | 1 | 0 | 0 | ||||
| CNR-925 | Streptomyces thermocoprophilus | 14 | 6 | FJ844682 | furA KS3 (S. aculeolatus), 1 | Actinofuranone | 96 | 1 | 1 | 0 | 0 |
| FJ844683 | furA KS2 (S. aculeolatus), 2 | Actinofuranone | 92 | 2 | 1 | 0 | 0 | ||||
| FJ844684 | furD KS1 (S. aculeolatus), 3 | Actinofuranone | 97 | 2 | 1 | 0 | 0 | ||||
| FJ844685 | tmnAI KS3 (Streptomyces sp. strain NRRL 11266), 4 | Tetronomycin | 97 | 3 | 1 | 0 | 0 | ||||
| FJ844686 | tmnAIV KS1 (Streptomyces sp. NRRL 11266), 5 | Tetronomycin | 97 | 4 | 1 | 0 | 0 | ||||
| FJ844687 | tmnAIV KS2 (Streptomyces sp. NRRL 11266), 6 | Tetronomycin | 98 | 4 | 1 | 0 | 0 | ||||
| CNR-927 | S. thermocoprophilus | 8 | 7 | FJ844688 | curA (Lyngbya majuscula) | Curacin | 52 | 1 | 1 | 0 | 0 |
| FJ844689 | curA (Lyngbya majuscula) | Curacin | 53 | 1 | 1 | 0 | 0 | ||||
| FJ844690 | ecoI (S. aizunensis) | ECO-02301 | 75 | 2 | 1 | 0 | 0 | ||||
| FJ844691 | ecoH (S. aizunensis) | ECO-02301 | 76 | 2 | 1 | 0 | 0 | ||||
| FJ844692 | ecoH (S. aizunensis) | ECO-02301 | 83 | 2 | 1 | 0 | 0 | ||||
| FJ844693 | jamL (Lyngbya majuscula) | Jamaicamide | 61 | 3 | 1 | 0 | 0 | ||||
| FJ844694 | vicD (S. halstedii) | Vicenistatin | 76 | 4 | 1 | 0 | 0 | ||||
| CNR-940 | S. thermocoprophilus | 35 | 6 | FJ844695 | ecoA (S. aizunensis) | ECO-02301 | 75 | 1 | 1 | 0 | 0 |
| FJ844696 | ecoA (S. aizunensis) | ECO-02301 | 74 | 1 | 1 | 0 | 0 | ||||
| FJ844697 | ecoA (S. aizunensis) | ECO-02301 | 78 | 1 | 1 | 0 | 0 | ||||
| FJ844698 | spnE (Saccharopolyspora spinosa) | Spinosad | 64 | 2 | 1 | 0 | 0 | ||||
| FJ844699 | spnE (Saccharopolyspora spinosa) | Spinosad | 73 | 2 | 1 | 0 | 0 | ||||
| FJ844700 | epoD (Polyangium cellulosum) | Epothilone | 59 | 3 | 1 | 0 | 0 | ||||
| Total | 79 | 8 | 3 | ||||||||
a
Top BLAST matches are to KS domains associated with experimentally characterized biosynthetic pathways. Clone identifiers correspond to those presented in Fig. 1 and 2.
b
KS amino acid sequences with ≥85% amino acid identity to the top BLAST matches are in bold.
c
Mod., modular; iter., iterative; hyb., hybrid.