TABLE 1.

PCR primers and FISH probes developed in this study

Primer or probe	Sequence (5′-3′)	Target, specificity	Temp (°C)a	% FAb
Primers
64F	GGA CGG TAA CAT TGT GGT GC	16S rRNA gene, X. bocki gammaproteobacterial endosymbiont	54
1417F	CGA AGG AGA GAC GGA GAA GGT TAG	23S rRNA gene, X. bocki gammaproteobacterial endosymbionts (sequencing primer)	59
Probes
Cps-1353modc	GAC GTT ATT GCT GAC ACG	16S rRNA, X. bocki chlamydial endosymbiont and relatives		15
XenoGam441	ATA GCC TTC CTC AGT GAT	16S rRNA, X. bocki gammaproteobacterial endosymbiont		15
XenoGam666	CGG AAA TTC CTC TAC CC	16S rRNA, X. bocki gammaproteobacterial endosymbiont and relatives		15
XenoGam727	TCC AGG TAG ACG CCT TC	16S rRNA, X. bocki gammaproteobacterial endosymbiont and relatives		15

^aTemp, applied primer annealing temperature for PCR.

^bFA, applied formamide concentration in the hybridization buffer for FISH.

^cModified from reference 28.