TABLE 1.
PCR primer sets used in this study
| Primer | Sequencea (5′ to 3′) | Gene target | Annealing temp (°C) | Amplicon size (bp) | Reference or source |
|---|---|---|---|---|---|
| Degen StxA | TTTGTYACYGTSAYAGCWGAAG | stx | 47.5 | ∼675 | 18 |
| Degen StxB | TYMTCATTATAYTTDGWRWACT | ||||
| Slt1-F | CGCTGTTGTACCTGGAAAGG | stx1 | 50.0 | ∼254 | 10 |
| Slt1-R | CGCTCTGCTGTGACAGTGAC | ||||
| Slt2-F | GCTTCTGCTGTGACAGTGAC | stx2 | 50.0 | ∼184 | 4 |
| Slt2-R | TCCATGACAACGGACAGCAG | ||||
| Q ATG 5′ | ATGTTCTTATGGTTCACCG | Lambdoid bacteriophage Q gene | 55.5 | ∼435 | 18 |
| Q 3′ | TTACGATCGTAAACTATTTTT | ||||
| GapA 3′ | CGCTCACATCTCCAAATAA | Bacterial gapA gene | 55.0 | ∼1,000 | This study |
| GapA 5′ | ATGACTATCAAAGTAGGTATC |
a
Ambiguities: K = G or T; S = G or C; W = A or T; Y = C or T; H = A, C, or T; R = A or G.