TABLE 3.
Putative functions of ORFs in cyclothiazomycin biosynthesis gene cluster
| Gene | Size of product (amino acids) | Homolog |
Putative function | % Identity | % Similarity | ||
|---|---|---|---|---|---|---|---|
| Protein | Organism | Accession no. | |||||
| cltA | 60 | Structural prepeptide | |||||
| cltB | 680 | Hypothetical protein | Actinomadura melliaura | ABC02784 | Cyclodehydratase | 47 | 57 |
| cltC | 562 | NADH oxidase | A. melliaura | ABC02783.1 | NADH oxidase (containing two McbC-like domains) | 36 | 47 |
| cltD | 647 | Hypothetical protein | A. melliaura | ABC02785.1 | Peptidase M14(N) YcaO family (C) | 28 | 41 |
| cltE | 875 | Lantibiotic dehydratase domain protein | Salinispora arenicola CNS-205 | ABV98429.1 | Lantibiotic dehydratase domain protein | 26 | 37 |
| cltF | 341 | Lantibiotic biosynthesis protein | Bacillus cereus ATCC 14579 | AAP11952.1 | Putative lantibiotic biosynthesis protein | 32 | 41 |
| cltG | 375 | Unknown | A. melliaura | ABC02780.1 | Unknown | 37 | 51 |
| cltH | 932 | NysRI | Streptomyces noursei | AF263912_17 | LuxR family transcriptional regulator | 30 | 40 |
| cltI | 192 | Putative transposase | Streptomyces ambofaciens ATCC 23877 | CAJ87970.1 | Putative transposase | 63 | 67 |
| cltJ | 170 | Epoxide hydrolase 1 | Streptomyces sp. strain Mg1 | EDX23590.1 | Epoxide hydrolase | 47 | 62 |
| cltM | 196 | Hypothetical protein | Streptomyces sp. strain FR1 | ABC67445.1 | Hypothetical protein | 44 | 58 |
| cltN | 111 | Ethyl tert-butyl ether degradation protein EthD | Caulobacter sp. strain K31 | ABZ74391.1 | Ethyl tert-butyl ether degradation protein EthD | 34 | 58 |
| cltO | 477 | Transport protein | Streptomyces coelicolor A3(2) | SCO2502 | Transport protein | 37 | 53 |
| cltP | 297 | DNA-binding protein | S. coelicolor A3(2) | SCO2501 | XRE family transcriptional regulator | 43 | 54 |
| cltQ | 116 | Putative transposase | S. coelicolor A3(2) | SCP1.215 | Putative transposase | 65 | 74 |