TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source or reference(s) |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk− mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Rita Shiang, Virginia Commonwealth University |
| E .coli BW25113 | K-12 derivative, ΔaraBAD ΔrhaBAD | 7, 12 |
| E. coli ET12567 | dam dcm hsdS cat tet | 5 |
| T. fusca(celR::neo) transformant | celR::neo | This study |
| Plasmids | ||
| pGEM-T | bla lacZ | Promega |
| pGEM2816 | bla celR lacZ | This study |
| pGEM2948neo | bla celR::neo lacZ | This study |
| pIJ790 | l RED (gam, bet, exo) cat araC, rep101ts | 12 |
| pET-30a(+) | neo lacI, T7 | Novagen |