TABLE 3.

Cellulase-related gene expression in wild-type and celR-disrupted T. fusca

Gene no.	Locus	Gene product (designation)	No. of 14-bp palindromes	Gene expression
				Wild-type activity (AU ± SD)	Mutant activity (AU ± SD)	P
1	Tfu_1627	β-1,4-Endoglucanase (Cel9B)	1 perfect	59.36 ± 1.68	98.14 ± 14.22	9.37E-03
2	Tfu_1074	β-1,4-Endoglucanase (Cel6A)	1 perfect	93.02 ± 5.28	29.27 ± 5.33	1.24E-04
3	Tfu_0620	β-1,4-Exocellulase (Cel6B)	2 perfect, 1 imperfect	34.94 ± 1.08	69.55 ± 5.47	4.24E-04
4	Tfu_2176	β-1,4-Endoglucanase (Cel9A), progressive	1 perfect	169.51 ± 17.82	456.57 ± 39.32	3.24E-04
5	Tfu_0901	Endo-1,4-β-glucanase (Cel5A)	1 perfect	51.85 ± 3.81	167.06 ± 22.29	9.10E-04
6	Tfu_1959	β-1,4-Exocellulase (Cel48A)	1 perfect, 5 imperfect	47.64 ± 3.27	86.48 ± 12.67	6.79E-03
7	Tfu_1268	Predicted cellulose-binding protein (E7)	None	82.08 ± 10.07	151.16 ± 18.34	4.63E-03
8	Tfu_1665	Predicted cellulose-binding protein (E8)	1 perfect	50.19 ± 2.63	65.21 ± 5.37	1.21E-02
9	Tfu_0934	Predicted ABC-type sugar transport system, periplasmic component for cellobiose/cellotriose	1 perfect	138.12 ± 4.02	39.57 ± 12.34	1.93E-04
10	Tfu_0937	β-Glucosidase (BglC)	None	335.90 ± 7.13	29.42 ± 5.35	4.76E-07
11	Tfu_0938	CelR	None	83.23 ± 5.17
12	Tfu_2923	β-1,4-Endoxylanase (Xyl10A)	1 imperfect	19.53 ± 1.69
13	Tfu_2788	Putative β-1,4-endoxylanase	None	80.57 ± 9.57	26.40 ± 4.11	8.41E-04
14	Tfu_1213	β-1,4-Xylosidase	None	33.40 ± 2.49	19.56 ± 3.56	5.27E-03
15	Tfu_2791	β-1,4-Endoxylanase (Xyl10B)	None	25.14 ± 1.04	24.26 ± 6.3	0.82
16	Tfu_1612	Putative secreted xyloglucanase	None	31.02 ± 2.29	7.46 ± 0.99	8.18E-05
17	Tfu_2990	Putative secreted xylanase	None	32.89 ± 1.19	8.26 ± 0.71	6.63E-06
18	Tfu_0900	Putative secreted β-mannanase	None	5.35 ± 0.23	12.14 ± 0.89	2.15E-04