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. 2010 Jan 29;76(7):2295–2303. doi: 10.1128/AEM.02462-09

FIG. 3.

FIG. 3.

Complementation of the BW25113ΔQAPRTase E. coli strain with the QAPRTase gene from either E. coli or mouse. The BW25113ΔQAPRTase strain was transformed with plasmids carrying the E. coli QAPRTase gene, the mouse QAPRTase gene, or no insert. Culture plates were inoculated with equal amounts of each bacterial strain and incubated for 12 h at 37°C. Three kinds of culture media, M9 medium containing NA (M9+NA), M9 medium containing NA and ampicillin (M9+NA+Amp), and M9 medium without any supplement, were used. The bacterial strains were as follows: I, BW25113; II, BW25113ΔQAPRTase; III-A, BW25113ΔQAPRTase/pUC19; III-B, BW25113ΔQAPRTase/pBAD-hisA; III-C, BW25113ΔQAPRTase/pCDNA3.1+; IV-A, BW25113ΔQAPRTase/pZJU11; IV-B, BW25113ΔQAPRTase/pZJU21; IV-C, BW25113ΔQAPRTase/pZJU31; V-A, BW25113ΔQAPRTase/pZJU12; V-B, BW25113ΔQAPRTase/pZJU22; V-C, BW25113ΔQAPRTase/pZJU32.