TABLE 1.
Sequencing best-match BLAST results for bands cut from DGGE gels and clones retrieved from library
| BLAST best match | % Similarity | No. of clones | Classification | Accession no. |
|---|---|---|---|---|
| Bands | ||||
| Tetrahymena sp. strain NI SSU rRNA gene, partial sequence (band of interest in Fig. 1) | 98 | NAa | Ciliophora, Hymenostomatida | GQ918140 |
| Epicarchesium abrae SSU rRNA gene, complete sequence (band 1, Fig. 2) | 97 | NA | Ciliophora, Peritrichia | GQ167353 |
| Spumella sp. strain 9-1-D1 SSU rRNA gene, partial sequence (band 2, Fig. 2) | 98 | NA | Heterokontophyta, Ochromonadales | GQ167354 |
| Zoothamnium duplicatum SSU rRNA gene, partial sequence (band 3, Fig. 2) | 96 | NA | Ciliophora, Peritrichia | GQ167355 |
| Hartmannella sp. strain 2 4/3Da/10 SSU rRNA gene, partial sequence (band 4, Fig. 2) | 92 | NA | Amoebozoa, Lobosea | GQ167356 |
| Epistylis galea SSU rRNA gene, complete sequence (band of interest in Fig. 5) | 95 | NA | Ciliophora, Peritrichia | GQ167357 |
| Clones | ||||
| Arcella hemisphaerica SSU rRNA gene, partial sequence | 93-99 | 11 | Amoebozoa, Lobosea | GQ167358-60, GQ167362, GQ167365-66, GQ167370-71, GQ167376-78 |
| Zoothamnium duplicatum SSU rRNA gene, partial sequence | 98-99 | 4 | Ciliophora, Peritrichia | GQ167364, GQ167374, GQ167384, GQ167386 |
| Chaos nobile CCAP 1511/2 SSU rRNA, partial sequence | 92 | 3 | Amoebozoa, Lobosea | GQ167363, GQ167372, GQ167375 |
| Cercomonadida environmental sample clone Elev_18S_776 SSU rRNA, partial sequence | 96 | 2 | Cercozoa, Cercomonadida | GQ167381, GQ167383 |
| Eimeriidae environmental sample clone Elev_18S_1405 SSU rRNA gene, partial sequence | 94-99 | 2 | Apicomplexa, Coccidia | GQ167373, GQ167382 |
| Epistylis urceolata SSU rRNA gene, complete sequence | 98 | 2 | Ciliophora, Peritrichia | GQ167369, GQ167385 |
| Euglypha acanthophora strain Millstream SSU rRNA gene, partial sequence | 100 | 1 | Amoebozoa, Lobosea | GQ167379 |
| Balamuthia mandrillaris isolate V451 SSU rRNA gene, partial sequence | 93 | 1 | Amoebozoa, Lobosea | GQ167367 |
| Stenamoeba sp. strain CRIB68 SSU rRNA gene, partial sequence | 94 | 1 | Amoebozoa, Thecamoebidae | GQ167380 |
| Acineta sp. strain OSW-2003-3 SSU rRNA gene, partial sequence | 90 | 1 | Ciliophora, Phyllopharyngea | GQ167368 |
| Uncultured eukaryote clone D4P08E02 SSU rRNA gene, partial sequence | 94 | 1 | NA | GQ167361 |
a
NA, not applicable.