TABLE 4.
Results of in silico analysis of primer specificity for NCBI RefSeq database sequences
| Primera | Representative species with 2 or fewer mismatches |
|---|---|
| cel48_490F | Clostridium spp. |
| cel48_920R | Anaerocellum, Bacillus, and Clostridium spp. |
| hydA_1290F | Clostridium, Coprococcus, Dehalococcoides, Desulfitobacterium, Geobacter, Marinitoga, Opitutus, Thermoanaerobacterium, Thermotoga, Tolumonas, and Veillonella spp. |
| hydA_1538R | Clostridium, Desulfovibrio, Fervidobacterium, Finegoldia, Fusobacterium, Marinitoga, Petrotoga, Thermodesulfovibrio, Thermosipho, and Thermotoga spp. |
| dsrA_290F | Desulfobotulus, Desulfomicrobium, Desulfomonile, Desulforhopalus, Desulfosalina, and Desulfovibrio spp. |
| dsrA_660R | Desulfobacter, Desulfobulbus, Desulfococcus, Desulfomicrobium, Desulfonema, Desulforhopalus, Desulfosalina, Desulfosarcina, Desulfotalea, and Desulfovibrio spp. |
a
In the case of the cel5 primer set (cel5_392F-cel5_754R), all reference sequences had more than 2 mismatches with the primer sequence.