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RT-PCR analysis of 31 selected SSH library genes. RNAs were extracted from conidia of the WT and ΔBbmpk1 mutant strains that were inoculated on cicada hind wings to induce appressorium formation (22 h after inoculation) and used for RT-PCR. All RT-PCR analysis was performed for 25 cycles using Bbgpd and β-tubulin as references. Lane 1, WT; lane 2, ΔBbmpk1 mutant.
