TABLE 1.
Bacterial strains used in this study
| Straina | Relevant genotypeb | Source or reference |
|---|---|---|
| W3110 | F− IN(rrnD-rrnE)1 λ− | ATCC 27325 |
| AH218 | BW25113 Δ(focA-pflB)-FRT-Km-FRT | 13 |
| AH240 | Δ(focA-pflB)-FRT-Km-FRT | W3110 × P1 (AH218) |
| AH241 | ΔldhA | 17 |
| BW25113 | lacIqrrnBT14 ΔlacZW116hsdR514 araBADAH33 ΔrhaBADLD78 | 6 |
| PMD23 | ΔhypF-FRT-Km-FRT | 15 |
| SE2378 | lpd101 ΔldhA Δ(focA-pflB)-FRT-Km-FRT pdhR | 17 |
| SE2382 | lpd101 ΔldhA Δ(focA-pflB)-FRT-Km-FRT | 18 |
| YK1 | lpd101 ΔldhA Δ(focA-pflB)-FRT pdhR Kms | SE2378; Kms |
| YK98 | BW25113 Δlpd-FRT-Km-FRT | This study |
| YK134 | Δlpd-FRT-Km-FRT | W3110 × P1 (YK98) |
| YK142 | lpd101 | YK134 × P1 (SE2382) |
| YK143 | ΔldhA Δlpd-FRT-Km-FRT | AH241 × P1 (YK98) |
| YK167 | lpd101 Δ(focA-pflB)-FRT-Km-FRT | YK142 × P1 (AH240) |
| QZ1 | ΔldhA lpd101 | YK143 × P1 (YK142) |
| QZ2 | lpd101 ΔhypF-FRT-Km-FRT | YK142 × P1 (PMD23) |
| QZ3 | ΔldhA lpd101 ΔhypF-FRT-Km-FRT | QZ1 × P1 (PMD23) |
a
All strains are derivatives of E. coli K-12 strain W3110, a nearly wild type strain deposited by J. Lederberg, unless noted otherwise (BW25113 and YK98).
b
FRT, FLP recombination target.