TABLE 4.
Anaerobic flux ratios at the pyruvate and acetyl-CoA branch points in select mutants with mutations in PDH, LDH, and/or PFL
| Strain | Enzyme(s)a | Anaerobic flux ratio at the following branch point: |
||||
|---|---|---|---|---|---|---|
| Pyruvate |
Acetyl-CoA |
|||||
| PFL | PDH | LDH | Acetate | Ethanol | ||
| PMD23 | PFL, LDH | 0.70 | 0.002 | 0.30 | 0.54 | 0.46 |
| QZ2 | PFL, LDH, PDH* | 0.66 | 0.01 | 0.33 | 0.55 | 0.45 |
| QZ3 | PFL, PDH* | 0.48 | 0.50 | 0.00 | 0.25 | 0.75 |
| YK167 | LDH, PDH* | 0.00 | 0.33 | 0.67 | 0.23 | 0.77 |
| SE2382 | PDH* | 0.00 | 1.00 | 0.00 | 0.22 | 0.78 |
| YK1 | PDH* | 0.00 | 1.00 | 0.00 | 0.15 | 0.85 |
a
Enzyme(s) expected to be functional at the pyruvate node in the indicated strain based on its genotype and phenotype and on the enzyme activities in the extract.