TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| Mach1 T1R | F− φ80lacZΔM15 ΔlacX74 hsdR(rK− mK+) ΔrecA1398 endA1 tonA | Invitrogen |
| TransforMax EC100D pir+ | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ−rpsL nupG pir+(DHFR) | Epicentre |
| HB101 | F−supE44 hsdS20(rB−mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi | 4 |
| X. citri subsp. citri strains | ||
| 306 | Rifr, causes citrus canker on citrus | 40 |
| D12 | galU::Tn5 derivative of strain 306, Rifr Knr | This study |
| F6 | galU::Tn5 derivative of strain 306, Rifr Knr | This study |
| CD | D12 containing pCGU2.1, Knr Cmr Gmr | This study |
| CF | F6 containing pCGU2.1, Knr Cmr Gmr | This study |
| Plasmids | ||
| pCR2.1 | PUC ori f1 ori lacZα+ Knr Apr | Invitrogen |
| pRK2013 | ColE1 Knr Tra+, conjugation helper plasmid | 14 |
| pUFR053 | IncW Mob+mob(P) lacZα+ Par+ Cmr Knr, shuttle vector | 15 |
| pCGU1.1 | galU gene from X. citri subsp. citri 306 cloned in pCR2.1 | This study |
| pCGU2.1 | galU gene on BamHI fragment from pCGU1.1 cloned in pUFR053 | This study |