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Primers used in this study
| Primer | Sequence (5′ → 3′)a |
|---|---|
| galU-F1 | AGACAGTGCCGAAAGAAATGCTGC |
| galU-R1 | AACAGCGATCAGGCAACAATCAGC |
| Kan2-F1 | CGAGGCCGCGATTAAATTCCAACA |
| Kan2-R1 | AGGCAGTTCCATAGGATGGCAAGA |
| KAN-2 FP-1 | ACCTACAACAAAGCTCTCATCAACC |
| R6KAN-2 RP-1 | CTACCCTGTGGAACACCTACATCT |
| CGU-F | AATGATGGATCCCTGCCAAAGCCTTGACGC |
| CGU-R | AACAGAGGATCCAACATCACCACGCCCAAC |
Underlined nucleotides are not exact matches and were introduced to add a BamHI restriction enzyme site.
