TABLE 3.
Genes and corresponding primers used for QRT-PCR analysis
| Gene | Product |
Primerb |
||
|---|---|---|---|---|
| Functiona | Component or protein | Directionc | Sequence (5′ → 3′) | |
| 16S rRNA | Ribosome component | F | CGCTTTCGTGCCTCAGTGTCAGTGTTGG | |
| R | GGCGTAAAGCGTGCGTAGGTGGTGGTT | |||
| hrpG | TTSS | TTSS regulator | F | GCCTTTCAATTCGCACGAGTTACACG |
| R | CACACGCCGGGGCTGGAAAAGA | |||
| hrpX | TTSS | TTSS regulator | F | AGCGATCTCTGCGTTGTCCTAC |
| R | ATACGCATCTTCGGCCTCTTCCTGA | |||
| hrcV | TTSS | TTSS component | F | GCGTTTGCGGCGTGCTTCATCT |
| R | CAATCTGGTGGTAGGCCTGGTCGTTTTCTT | |||
| pthA | TTSS | TTSS effector | F | TGGCGTCGGCAAACAGTGGTC |
| R | TGCTCCGGGGTCAGGTTCAGG | |||
| avrBs2 | TTSS | Avirulence protein | F | CGCGCCAATCACGACAAGGACTACTAC |
| R | CGGGCCAGCGTGCGGTTTTC | |||
| avrXacE1 | TTSS | Avirulence protein | F | TCGCGCTGGGCCGGAACATACC |
| R | GCGTCCGCGGCGATAACTCTTG | |||
| XAC0028 | CWDE | Cellulase | F | CGCTCCACGCTGCTTTTCAT |
| R | ATTCGGCACCGGACAGATTG | |||
| XAC0160 | CWDE | Xylanase | F | CATGGCCTGGCGGTCCTTGTGCT |
| R | GCGCGATCCGGCTGGCTTGTTC | |||
| XAC0165 | CWDE | Xylosidase | F | AGGGCGGGGCGTTGCTGTTCTAC |
| R | TCTTGCCGTCGGGACTGCTGTGA | |||
| peh-1 | CWDE | Endopolygalacturonase | F | AGTGGCAACGCGTTTCTGACC |
| R | CGCCTGCGTTGTTGCCCTTGAC | |||
| celD | CWDE | Glucan 1,4-beta-glucosidase | F | GATTTCGGCCGAGCGTCTGGA |
| R | GGATGCCGGCCTGGTTCTTCA | |||
| pglA | CWDE | Polygalacturonase | F | CAGCGCCGACGTCACCTTGTA |
| R | GTAGCCGGGACGCGAATAGACC | |||
| pelB | CWDE | Pectate lyase II | F | GAACTTCGGCGTGCGTGTGGTG |
| R | GTGAGCGAGGCGAAGATGGTGTTGTGGTC | |||
| gumB | EPS synthesis | Polysaccharide exporter | F | CTGACCGAAATCGAGAAGGGCACCAATC |
| R | GCGCCACACCATCACAAGAGGAGTCAGTTC | |||
| kpsF | CPS synthesis | Arabinose 5-phosphate isomerase | F | GCTTCACCGCCGACGACTTC |
| R | CGCTTGCGGCTCATTTCCATC | |||
a
TTSS, type III secretion system; CWDE, cell wall-degrading enzyme; EPS, extracellular polysaccharides; CPS, capsular polysaccharide.
b
The primers were derived from the sequences of corresponding genes.
c
F, forward; R, reverse.