TABLE 4.

Comparison of expression of key virulence genes in the wild type and the galU mutant grown in XVM2 medium using QRT-PCR

Gene	Mean ΔΔCTa	SD	95% Confidence interval	P	Change (fold)b
hrpG	−1.6804	0.5275	−2.3590, −1.0018	0.0003c	3.2052
hrpX	−2.1666	0.5206	−2.8363, −1.4968	<0.0001c	4.4896
hrcV	−2.0417	0.4961	−2.6799, −1.4035	<0.0001c	4.1173
pthA	−0.2077	0.3894	−0.7086, 0.2933	0.3774	1.1548
avrBs2	−2.1722	0.6046	−2.9499, −1.3944	<0.0001c	4.5071
avrXacE1	−2.4682	1.0118	−3.7699, −1.1665	0.0018c	5.5335
XAC0028	2.2138	0.7257	1.2802, 3.1474	0.0004c	0.2156
XAC0160	0.5413	1.3596	−1.2078, 1.2903	0.5062	0.6872
XAC0165	1.2379	0.3362	0.8054, 1.6705	<0.0001c	0.4240
peh-1	−2.8464	0.9095	−4.0164, −1.6764	0.0003c	7.1920
celD	0.4748	1.0723	−0.9047, 1.8542	0.4609	0.7196
pglA	−0.5460	0.8444	−1.6322, 0.5402	0.2889	1.4600
pelB	1.2397	0.2500	0.9182, 1.5613	<0.0001c	0.4235
gumB	−0.9043	0.5975	−1.6730, −0.1356	0.0255c	1.8716
kpsF	−0.3704	0.9612	−1.6069, 0.8661	0.5196	1.2927

^aThe mean ΔΔC_T was determined using six biological replicates.

^bThe change in expression in D12 was calculated using .

^cValues are significantly different when P is <0.05.