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Quantification of biofilm. Biofilms of wild-type strain ES5 and mutants were measured using the CV microtiter assay. The values are means from at least two independent experiments with 2 to 8 replicates in each experiment. The error bars indicate one standard deviation. The dashed line indicates the wild-type strain ES5 level (100%). All mutant values were significantly different from the wild-type value (P < 0.001, ranked Welch test). wt, wild type.
