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. 2010 Jan 29;76(7):2251–2261. doi: 10.1128/AEM.00930-09

FIG. 3.

FIG. 3.

Complementation of mutants BF14 and BF15. Quantification of biofilm for wild-type strain ES5, strain ES5(pUC18NotI), mutants BF14 (ESA_00281::Tn) and BF15 (ESA_00282::Tn), and complemented mutants in the CV microtiter assay. The values are means of values from at least three independent experiments with at least six replicates in each experiment. The error bars indicate one standard deviation. The dashed line indicates the ES5(pUC18NotI) level. All values were compared to the wild-type level, and one asterisk indicates that there is a significant difference (P < 0.05, Welch test). BF14 (pUC18NotI), BF14(pP1b), BF15(pUC18NotI), and BF15(pR8b) were also compared to ES5(pUC18NotI). Significant differences (P < 0.01, Welch test) are indicated by two asterisks. wt, wild type.