FIG. 4.
CLSM image stack profiles of flow cell biofilms. Each large panel shows a horizontal cross section of a flow cell-grown biofilm, while the smaller panels show vertical cross sections. The dashed lines in the panels indicate the planes of the other two cross sections. (A) C. sakazakii strain wild-type strain ES5 at 24 h and 45 h after start of incubation. BL, basal layer; MC, microcolony. (B) Mutants BF14 (ESA_00281::Tn) and BF15 (ESA_00282::Tn) and complemented mutants BF14 and BF15 at 45 h after the start of incubation. All images were taken at a magnification of ×400.