TABLE 3.
Primers used in this study
| Primer | Nucleotide sequence (5′→3′)a | Reference or source |
|---|---|---|
| 280r4 | CGG GCC AAG CTT GAA CGC TGG ATC AGG TGA | This study |
| 281f1 | CCG GGC AAG CTT TAC TGT TCA TGC GCA GGC | This study |
| 282f2 | CCG GGC AAG CTT GGA ATA CGA TAA CGC GCC | This study |
| 282r1 | CGG GGC AAG CTT AAG CGT TCC ATA GCG TCC | This study |
| KAN-2 FP1 | ACC TAC AAC AAA GCT CTC ATC AAC C | Epicentre |
| M13 | TGT AAA ACG ACG GCC AG | New England Biolabs |
| M13r | CAG GAA ACA GCT ATG ACC | New England Biolabs |
| Tn5PCRF | GCT GAG TTG AAG GAT CAG ATC | 44 |
a
Recognition sequences of restriction enzymes are underlined.