Figure 6.

Loss of kinesin-1 from APP transport vesicles in Rab3GAP p130 knock-out mice. Low-density membrane fractions from wild-type (wt) or Rab3GAP p130 knock-out mouse (p130^−/−) brain homogenates were immunoisolated with anti-APP antibodies (IS:APP) and subjected to Western blot analyses using antibodies directed against Rab3GAP p130, Rab3GAP p150, kinesin-1, Rab3, and two putative cargoes of APP transport vesicles, RIM2 and Munc13-1 (see Fig. 1). Total lysate ($\raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$80$}\right.$) from each brain was loaded in the lanes of the left panel as input control (Input).