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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Int J Cancer. 2009 Aug 15;125(4):816–825. doi: 10.1002/ijc.24347

Figure 2. Three-dimensional collagen I culture downregulated CTGF in many cell types.

Figure 2

Breast carcinoma MDA-MB231 (A), rat cortical neurons (B), fibrosarcoma HT1080 (C), and HUVEC (D) were cultured atop 3DCI and on thin layer collagen I for indicated periods of time. Real time RT-PCR was performed to evaluate CTGF mRNA expression. Ratio of CTGF mRNA expression on 2DCI versus 3DCI was quantified with 2-ΔΔCt method. Ratio equal to 1 represents no change, greater than 1 denotes an increase, and lower than 1 shows a decrease between the compared conditions. Experiments for each cell type were performed twice, averaged and plotted as mean ± standard deviation, *, p<0.05.