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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Clin Cancer Res. 2009 Aug 25;15(17):5338–5349. doi: 10.1158/1078-0432.CCR-08-2865

Figure 3. Transcript-b levels are refractory to androgen and AR activity.

Figure 3

A). To assess AR activity on cyclin D1 transcript-a and transcript-b levels, LNCaP cells were cultured in either in the absence of steroid hormones (charcoal dextran treated serum, CDT) and stimulated 24 hrs with (or not) with 1 nM DHT, as indicated, or in complete serum (5% FBS) supplemented where indicated with 1 uM Casodex. Real time PCR for relative KLK3/PSA (upper panel), transcript-a (middle panel), transcript-b (lower panel) are shown. Representative images are shown in Supplemental Figure S3. B). To evaluate the contribution of mTOR-mediated post-transcriptional regulation of cyclin D1b, LAPC4 cells were treated with 10 uM RAD001 for 4 hrs. Cyclin D1b levels were determined by immunoblot analysis using the cyclin D1b-specific antibody with lamin (control). Representative immunoblot is shown.