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Immunoblotting to determine antibody reactivity to rBipA and borrelia lysates. The serum samples used included pooled immune sera from three mice (A) and three human patients infected with B. hermsii (B to D) or two human patients infected with B. burgdorferi (E and F). Arrows indicate protein bands of the expected size for rBipA, and the asterisk indicates the expected size of native BipA in B. hermsii DAH lysates. Molecular mass standards are indicated in kilodaltons.
