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. 2010 Feb 10;17(4):564–571. doi: 10.1128/CVI.00518-09

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FIG. 4. — Immunoblotting with pooled sera from mice immunized with rBipA. Immune sera against rBipA (A) and preimmunization sera (B) were used to determine seroconversion to BipA in B. hermsii lysates. Five species of Borrelia (C), GGI (D), and GGII (E) isolates of B. hermsii were also probed with anti-rBipA sera (C to E, top panels) and a monoclonal antibody generated against B. hermsii flagellin (C to E, lower panels). Molecular mass standards are indicated on the left of each immunoblot in kilodaltons.