FIG. 1.

Changes in CD4 and CD8 T-cell subsets expressing markers for T_EM and T_CM in target organs over the course of the study. (A) Representative gating strategy to identify and quantitate CD62L^lo CCR7^lo CD44^hi T_EM and CD62L^hi CCR7^hi CD44^hi T_CM populations. (B) Numbers of CD4 T_EM, CD8 T_EM, CD4 T_CM, and CD8 T_CM in the lungs versus time of the infection. Test groups were mice chronically infected with M. tuberculosis (▪), mice that had been subcutaneously vaccinated with BCG (▴), or age-matched uninfected controls (▾). In each case, the data point represents the log₁₀ mean value for cells recovered from the lungs or other organs of four or five mice; standard errors of the means (SEM) are omitted for clarity and did not exceed 10%. In all cases, numbers of cells were increased in the infected animals compared to uninfected age-matched controls (for M. tuberculosis infection at all time points, P < 0.01 or lower for T_EM values and P < 0.02 for T_CM values; for BCG, P < 0.04 [day 40 CD4 and CD8 T_EM] and P < 0.03 or lower [for all other data points]).