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. 2010 Feb 24;17(4):596–601. doi: 10.1128/CVI.00320-09

FIG. 1.

FIG. 1.

Illustration of imprecision in the reactive spot counts in the ELISPOT images. The sample was picked up from our routine analysis. (Upper panel) No reactivity in culture media (Blank, negative control of the kit), with one or two reactive spots under stimulations with antigen A (Panel A). (Lower panel) Reactivities of six spots under stimulation with antigen B (positive per manufacturer) and three spots in a replicate well (negative per manufacturer) (Panel B) as well as reactivities to PPD and PHA (positive control of the kit).