TABLE 1.
Within-run imprecisions of B-TbIFNg and Ly-TbSpot
| Sample | Method (no. of replicates) | Detected IFN-γ responsea |
CV% | |
|---|---|---|---|---|
| Mean (range) | SD | |||
| 1 | B-TbIFNg (10) | 2.7 (2.48-2.86) IU/ml | 0.11 IU/ml | 4.4 |
| Ly-TbSpot with AgA (8) | 227 (165-290) SFU/106 lymph | 40 SFU/106 lymph | 17.5 | |
| Ly-TbSpot with AgB (8) | 310 (267-358) SFU/106 lymph | 29 SFU/106 lymph | 9.4 | |
| 2 | B-TbIFNg (6) | 97 (93-102) IU/ml | 2.95 SFU/106 lymph | 3.0 |
| Ly-TbSpot with AgA (6) | 901 (866-931) SFU/106 lymph | 28 SFU/106 lymph | 3.1 | |
| Ly-TbSpot with AgB (6) | 540 (455-668) SFU/106 lymph | 73 SFU/106 lymph | 13.5 | |
a
SFU/106 lymph, spot-forming units per million lymphocytes.