Skip to main content
. 2010 Feb 24;17(4):631–637. doi: 10.1128/CVI.00511-09

FIG. 2.

FIG. 2.

Effect of antigen concentration on reactivity in the ES33- and T24-MICTs. (A) Different concentrations of rES33 were tested to determine the optimum signal-to-noise ratio of the MAR value for the ES33-MICT. MAR value, the ratio of the RMU of test line divided by the RMU of control line; S/N, signal-to-noise ratio (the MAR value of the positive serum divided by the MAR value of the negative serum); N, negative serum; L, low-positive serum; M, medium-positive serum; 0.06, 1 mg/ml of rES33 applied at 0.06 μl/mm; 0.08, 1 mg/ml of rES33 applied at 0.08 μl/mm; 0.1, 1 mg/ml of rES33 applied at 0.1 μl/mm; 0.8-0.08, 0.8 mg/ml of rES33 applied at 0.08 μl/mm. (B) Different concentrations of rT24H were used to determine the optimum signal-to-noise ratio of the MAR value for the T24-MICT. 1.25, 2.5 mg/ml of rT24H applied at 0.05 μl/mm; 1.875, 2.5 mg/ml of rT24H applied at 0.075 μl/mm; 2.5, 2.5 mg/ml of rT24H applied at 0.1 μl/mm; 3, 2.5 mg/ml of rT24H applied at 1.25 μl/mm; 3.75, 2.5 mg/ml of rT24H applied at 1.5 μl/mm.